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ppb pgk destination 150ng  (Addgene inc)


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    Structured Review

    Addgene inc ppb pgk destination 150ng
    Ppb Pgk Destination 150ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppb+pgk+destination/pPB-PGK-destination+(Plasmid+%2360436)/bio_rxiv__2025__11__14__688451-60-14-18
    Average 93 stars, based on 4 article reviews
    ppb pgk destination 150ng - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancer.
    Article Snippet: Colonies containing the entry vector were miniprepped using the MonarchTM Plasmid Miniprep Kit (NEB, T1010S). .. Next, the entry vector was reacted with pPB-PGK-Destination (Addgene # 60436) using LR Clonase II (Thermo Scientific,11791020) to generate the desired expression vector. .. The sequencing validated PB-ER-SIINFEKL construct was co-electroporated along with the Super PiggyBac transposase (System Biosciences, PB210PA-1) into parental E0771 mouse breast cancer cells (CH3 BioSystems) using the Neon Transfection system (Life Technologies, InvitrogenTMMPK5000).

    Article Title: Patient-derived and gene-edited pluripotent stem cells lacking NPHP1 recapitulate juvenile nephronophthisis in abnormalities of primary cilia and renal cyst formation
    Article Snippet: To clone the human NPHP1 CDS (CoDing Sequence), total RNA was extracted from hiPSC line 1383D6 with FastGene RNA premium kit (Nippon Genetics). cDNA was synthesized by RT-PCR using ReverTra Ace (TOYOBO). .. For In-Fusion cloning, two primer sets with 15 bp overlapping regions were designed for PCR, producing a cDNA fragment of NPHP1 and linearized vector of pPB-PGK-destination (#60436, Addgene). ..

    Expressing:

    Article Title: Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancer.
    Article Snippet: Colonies containing the entry vector were miniprepped using the MonarchTM Plasmid Miniprep Kit (NEB, T1010S). .. Next, the entry vector was reacted with pPB-PGK-Destination (Addgene # 60436) using LR Clonase II (Thermo Scientific,11791020) to generate the desired expression vector. .. The sequencing validated PB-ER-SIINFEKL construct was co-electroporated along with the Super PiggyBac transposase (System Biosciences, PB210PA-1) into parental E0771 mouse breast cancer cells (CH3 BioSystems) using the Neon Transfection system (Life Technologies, InvitrogenTMMPK5000).

    Cloning:

    Article Title: Patient-derived and gene-edited pluripotent stem cells lacking NPHP1 recapitulate juvenile nephronophthisis in abnormalities of primary cilia and renal cyst formation
    Article Snippet: To clone the human NPHP1 CDS (CoDing Sequence), total RNA was extracted from hiPSC line 1383D6 with FastGene RNA premium kit (Nippon Genetics). cDNA was synthesized by RT-PCR using ReverTra Ace (TOYOBO). .. For In-Fusion cloning, two primer sets with 15 bp overlapping regions were designed for PCR, producing a cDNA fragment of NPHP1 and linearized vector of pPB-PGK-destination (#60436, Addgene). ..

    Polymerase Chain Reaction:

    Article Title: Patient-derived and gene-edited pluripotent stem cells lacking NPHP1 recapitulate juvenile nephronophthisis in abnormalities of primary cilia and renal cyst formation
    Article Snippet: To clone the human NPHP1 CDS (CoDing Sequence), total RNA was extracted from hiPSC line 1383D6 with FastGene RNA premium kit (Nippon Genetics). cDNA was synthesized by RT-PCR using ReverTra Ace (TOYOBO). .. For In-Fusion cloning, two primer sets with 15 bp overlapping regions were designed for PCR, producing a cDNA fragment of NPHP1 and linearized vector of pPB-PGK-destination (#60436, Addgene). ..

    other:

    Article Title: Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancer.
    Article Snippet: The sequencing validated PB-ER-SIINFEKL construct was co-electroporated along with the Super PiggyBac transposase (System Biosciences, PB210PA-1) into parental E0771 mouse breast cancer cells (CH3 BioSystems) using the Neon Transfection system (Life Technologies, InvitrogenTMMPK5000).



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